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KMID : 0624620200530050254
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BMB Reports
2020 Volume.53 No. 5 p.254 ~ p.259
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MiR-449a attenuates autophagy of T-cell lymphoma cells by downregulating ATG4B expression
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Zhang Nan
Qiu Ling
Li Tao
Wang Xiao
Deng Rui
Yi Hai
Su Yi
Fan Fang-Yi
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Abstract
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Increasing evidence suggests the role of miR-449a in the regulation of tumorigenesis and autophagy. Autophagy plays an important role in the malignancy of T-cell lymphoma. However, it is still unknown whether miR-449a is associated with autophagy to regulate the malignancy of T-cell lymp homa. In this study, we for the first time demonstrated that miR-449a enhanced apoptosis of T-cell lymphoma cells by decreasing the degree of autophagy. Further, miR-449a downregulated autophagy- associated 4B (ATG4B) expression, which subsequently reduced the autophagy of T-cell lymphoma cells. Mechanistically, miR-449a decreased ATG4B protein level by binding to its mRNA 3’UTR, thus reducing the mRNA stability. In addition, studies with nude mice showed that miR-449a significantly inhibited lymphoma characteristics in vivo. In conclusion, our results demonstrated that the “miR-449a/ATG4B/autophagy” pathway played a vital role in the malignancy of T-cell lymphoma, suggesting a novel therapeutic target.
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KEYWORD
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ATG4B, Autophagy, MiR-449a, Post-transcriptional regulation, T-cell lymphoma
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